THE REDUCTION
OF E. COLI ON VARIOUS COUNTERTOP SURFACES
O. Peter Snyder, Jr., Ph.D.
Hospitality Institute of Technology and Management
March 22, 1999
Introduction
The purpose of this study was to determine the cleanability
of six countertop surfaces.
1. Laminate
2. Wood
3. Tile
4. Concrete
5. Stainless steel
6. Granite
These materials are commonly used in home kitchen countertop
construction. Today, it is understood that many food items
that we purchase are highly contaminated with pathogenic
microorganisms, and it is necessary for the home cook to
make these foods safe. Often, the first step in food preparation
is cutting and manipulating the food to get it ready. It
is essential that the countertop be cleaned after raw food
has touched the surface. Otherwise, there can be cross-contamination,
and the people eating the food prepared on the cross-contaminated
surface can become ill. This study identifies the cleanability
of these six different countertop materials.
Methods
The countertop materials were supplied by Porter Novelli
(1120 Connecticut Avenue NW; Washington, DC 20036-3902).
The countertop sources are as follows.
1. Laminate: Wilson Art #4557-60; color-dakota
ridge
2. Wood: maple, class 65 woods
3. Tile: Daytona tile, grade 5 (no stock information;
made in Italy); ceramic clay tile fired from 9-1,300ºC
with a single glaze
4. Concrete: custom sample; no specific information
5. Stainless steel: type 304, number 4 finish
6. Granite: custom sample; Lelajaross, 2-cm-thick sample
The procedure for doing the experiments was as follows.
E. coli ATTC# 25922, a non-pathogenic E. coli,
was used as the marker organism. It was grown overnight
at 35ºC in a static culture of M broth (Intermational
Bioproducts; 14780 NE 95th Street, Redmond, WA 98052) to
an inoculum of approximately 1,000,000,000 organisms per
ml.
An area of 81 square inches of each countertop was inoculated
with 1 ml of this culture in M broth. The cleaning procedure
was as follows. First, the surface was washed with a dishcloth
and 2 liters of detergent (Jefco Yellow Dishsoap; Unisource
/ Jefco Group, Inc.; 1040 North Halsted Street; Chicago,
IL 60622) water in a stainless steel bowl. The surface
was then rinsed, using a second bowl with 2,000 ml of clear
water and a second dishcloth. The cleaning process entailed
rubbing the dishcloth left to right over the surface, rinsing
it out, then, rubbing up and down and rinsing it out. The
rinse step followed the wash step, using the same left-to-right
and up-and-down strokes.
After the surfaces were washed and rinsed, they were swabbed,
using a sponge swab over the entire 81 square inches of
inoculated surface to find the mean reduction. The sponge
swab was cultured using Violet Red Bile agar plates (International
Bioproducts) and incubated overnight at 35ºC.
Following the wash and rinse, the surfaces were wiped
with a 10% solution of white household vinegar (1 cup 5%
vinegar in 9 cups tap water). The surfaces were allowed
to dry for 15 minutes. They were then sponge-swabbed over
the 81 square inches once more, and cultured, using VRB
agar to determine how many E. coli had been destroyed
by the vinegar.
Results
The results of the experiment are shown in Table 1 as
logarithms of counts per 81 square inches of surface. In
the column, "Real number," the logarithms of the average
are converted to real numbers. In each experiment, the
first column presents the log mean count of the organisms
recovered. The second column under each experiment shows
the log reduction in bacteria due to the wash-and rinse
process or due to the vinegar application. The results
are also shown in Figure 1.
The retention of the E. coli was from most retained
to most removed as follows.
1. Laminate
2. Wood
3. Tile
4. Concrete
5. Stainless steel
6. Granite
For the laminate, washing and rinsing reduced the
bacterial counts by about 285 to 1, as shown in the summary
column. When the vinegar was applied, the overall reduction
was increased to about 500,000 to 1.
For the wood, washing and rinsing reduced the bacterial
counts by about 500 to 1, as shown in the summary column.
When the vinegar was applied, the overall reduction was
increased to about 2,000 to 1.
For the tile, washing and rinsing reduced the bacterial
counts by about 900 to 1, as shown in the summary column.
When the vinegar was applied, the overall reduction was
increased to about 233,000 to 1.
For the concrete, washing and rinsing reduced the
bacterial counts by about 2,400 to 1, as shown in the summary
column. When the vinegar was applied, the overall reduction
was increased to about 30,600 to 1.
For the stainless steel, washing and rinsing reduced
the bacterial counts by about 4,000 to 1, as shown in the
summary column. When the vinegar was applied, the overall
reduction was increased to about 230,000,000 to 1.
For the granite, washing and rinsing reduced the
bacterial counts by about 36,000 to 1, as shown in the
summary column. When the vinegar was applied, the overall
reduction was increased to about 80,000,000 to 1.
Discussion
This experiment has shown that every countertop will have
a different cleanability. This experiment was done with
new samples. When some of these samples become worn, the
reduction will probably not be as significant, except for
stainless steel, which should change the least.
While granite showed the greatest reduction in washing,
overall, after the vinegar sanitizing, the stainless steel
had the greatest reduction.
Conclusion
It is very important in food safety for the designer to
consider the countertop material. In this case, the stainless
steel counter showed the greatest overall reduction after
the wash, rinse, and sanitize processes.
Table 1. Log Reduction
Comparison of Non-pathogenic Escherichia coli on
Surfaces [CFU / 81 sq. in.] as Affected by Surface Composition,
Washing and Rinsing, and Sanitizing with Vinegar
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Expt. 1
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Expt. 2
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Expt. 3
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Expt. 4
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Expt. 5
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Summary
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Material
|
Trmt.
|
Mean
|
Rdtn.
|
Mean
|
Rdtn.
|
Mean
|
Rdtn.
|
Mean
|
Rdtn.
|
Mean
|
Rdtn.
|
Log avg. rdtn.
|
Numerical value
|
Std. dev. of log av. rdtn.
|
| Innoculum |
9.18 |
|
9.33 |
|
9.34 |
|
9.25 |
|
9.26 |
|
9.27 |
1,862,087,137 |
0.07 |
| Laminate |
W & R |
4.85 |
4.33 |
6.38 |
2.95 |
6.25 |
3.09 |
8.35 |
0.90 |
8.25 |
1.01 |
2.46 |
286 |
1.32 |
| |
Vinegar |
3.65 |
5.53 |
3.43 |
5.90 |
3.25 |
6.09 |
3.84 |
5.41 |
3.70 |
5.56 |
5.70 |
498,884 |
0.25 |
| Wood |
W & R |
6.38 |
2.80 |
6.89 |
2.44 |
6.76 |
2.58 |
6.62 |
2.63 |
6.55 |
2.71 |
2.63 |
429 |
0.12 |
| |
Vinegar |
5.82 |
3.36 |
6.00 |
3.33 |
5.98 |
3.36 |
6.04 |
3.21 |
5.93 |
3.33 |
3.32 |
2,080 |
0.06 |
| Tile |
W & R |
6.23 |
2.95 |
6.19 |
3.14 |
6.41 |
2.93 |
6.34 |
2.91 |
6.39 |
2.87 |
2.96 |
912 |
0.09 |
| |
Vinegar |
4.33 |
4.85 |
3.28 |
6.05 |
2.30 |
7.04 |
4.51 |
4.74 |
4.60 |
4.66 |
5.47 |
293,765 |
0.94 |
| Concrete |
W & R |
5.81 |
3.37 |
5.89 |
3.44 |
6.29 |
3.05 |
5.80 |
3.45 |
5.69 |
3.57 |
3.38 |
2,377 |
0.18 |
| |
Vinegar |
4.28 |
4.9 |
4.73 |
4.60 |
4.85 |
4.49 |
4.92 |
4.33 |
5.00 |
4.26 |
4.52 |
32,810 |
0.23 |
| Granite |
W & R |
4.86 |
4.32 |
4.26 |
5.07 |
4.76 |
4.58 |
4.78 |
4.47 |
4.93 |
4.33 |
4.55 |
35,810 |
0.28 |
| |
Vinegar |
0 |
9.18 |
1.24 |
8.09 |
0 |
9.34 |
2.88 |
6.37 |
2.74 |
6.52 |
7.90 |
79,432,823 |
1.26 |
| Stain.Stl. |
W & R |
5.22 |
3.96 |
6.28 |
3.05 |
5.84 |
3.5 |
5.48 |
3.77 |
5.55 |
3.71 |
3.60 |
3,963 |
0.31 |
| |
Vinegar |
0 |
9.18 |
1.30 |
8.03 |
3.26 |
6.08 |
0 |
9.25 |
2.15 |
7.11 |
7.93 |
85,113,804 |
1.22 |
Rdtn = reduction 0 = <100
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